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primary antibody against cd14  (Servicebio Inc)

 
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    Servicebio Inc primary antibody against cd14
    Hypoxia-response TAMs in GBM. (A) UMAP visualization of TAMs. (B) UMAP visualization showing TAMs of individuals. (C) Top 20 function analysis results of TAM-1 cluster. Hypoxia-related biological processes were colored in red. (D) Violin plot of ERO1A expression level in different TAM clusters. (E) Immunofluorescence staining for TAM-1 cluster ( <t>CD14+ERO1A+</t> ) in patient tumor sample. The staining was performed for seven patients, one section each, and a representative image from patient 6 with TAM-1 pointed out by white arrows was shown; scale, 10 μm. The other images are shown in Supplementary <xref ref-type= Supplementary Figure 6 . (F) Composition ratio of the TAM clusters in individual GBM. (G) Correlation between the number of MES-like and TAM-1 cells. (H) Heatmap of specific gene sets in TAMs. (I) Violin plot of the hypoxia gene set scores in TAMs. TAM-1 was the hypoxia-response cluster in TAMs. (J) The activated regulons in the TAM-1 cluster ranked by the regulon specificity score from high to low. (K) Survival analysis of the TAM-1 cluster signatures in TCGA and CGGA GBM databases, respectively. Gray dash line, average score; **** p < 0.0001. See also Supplementary Figures 5 and 6 , and Supplementary Tables 3 and 6 . " width="250" height="auto" />
    Primary Antibody Against Cd14, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibody against cd14/product/Servicebio Inc
    Average 90 stars, based on 1 article reviews
    primary antibody against cd14 - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Single-Cell Transcriptomics Revealed Subtype-Specific Tumor Immune Microenvironments in Human Glioblastomas"

    Article Title: Single-Cell Transcriptomics Revealed Subtype-Specific Tumor Immune Microenvironments in Human Glioblastomas

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2022.914236

    Hypoxia-response TAMs in GBM. (A) UMAP visualization of TAMs. (B) UMAP visualization showing TAMs of individuals. (C) Top 20 function analysis results of TAM-1 cluster. Hypoxia-related biological processes were colored in red. (D) Violin plot of ERO1A expression level in different TAM clusters. (E) Immunofluorescence staining for TAM-1 cluster ( CD14+ERO1A+ ) in patient tumor sample. The staining was performed for seven patients, one section each, and a representative image from patient 6 with TAM-1 pointed out by white arrows was shown; scale, 10 μm. The other images are shown in Supplementary <xref ref-type= Supplementary Figure 6 . (F) Composition ratio of the TAM clusters in individual GBM. (G) Correlation between the number of MES-like and TAM-1 cells. (H) Heatmap of specific gene sets in TAMs. (I) Violin plot of the hypoxia gene set scores in TAMs. TAM-1 was the hypoxia-response cluster in TAMs. (J) The activated regulons in the TAM-1 cluster ranked by the regulon specificity score from high to low. (K) Survival analysis of the TAM-1 cluster signatures in TCGA and CGGA GBM databases, respectively. Gray dash line, average score; **** p < 0.0001. See also Supplementary Figures 5 and 6 , and Supplementary Tables 3 and 6 . " title="... clusters. (E) Immunofluorescence staining for TAM-1 cluster ( CD14+ERO1A+ ) in patient tumor sample. The staining was ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Hypoxia-response TAMs in GBM. (A) UMAP visualization of TAMs. (B) UMAP visualization showing TAMs of individuals. (C) Top 20 function analysis results of TAM-1 cluster. Hypoxia-related biological processes were colored in red. (D) Violin plot of ERO1A expression level in different TAM clusters. (E) Immunofluorescence staining for TAM-1 cluster ( CD14+ERO1A+ ) in patient tumor sample. The staining was performed for seven patients, one section each, and a representative image from patient 6 with TAM-1 pointed out by white arrows was shown; scale, 10 μm. The other images are shown in Supplementary Supplementary Figure 6 . (F) Composition ratio of the TAM clusters in individual GBM. (G) Correlation between the number of MES-like and TAM-1 cells. (H) Heatmap of specific gene sets in TAMs. (I) Violin plot of the hypoxia gene set scores in TAMs. TAM-1 was the hypoxia-response cluster in TAMs. (J) The activated regulons in the TAM-1 cluster ranked by the regulon specificity score from high to low. (K) Survival analysis of the TAM-1 cluster signatures in TCGA and CGGA GBM databases, respectively. Gray dash line, average score; **** p < 0.0001. See also Supplementary Figures 5 and 6 , and Supplementary Tables 3 and 6 .

    Techniques Used: Expressing, Immunofluorescence, Staining



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    (A) Quantitative marker analysis showing percent of cells expressing CD73, <t>CD105,</t> CD14 and CD45 after long term expansion Synthemax II microcarriers in Mesencult XF and hMSC stemgro media. Flow cytometry histograms for each phenotypic marker are shown for cells maintained on Synthemax II microcarriers in Mesencult XF medium (B) and hMSC stemgro medium (C).
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    primary antibody against cd14  (Servicebio Inc)
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    Hypoxia-response TAMs in GBM. (A) UMAP visualization of TAMs. (B) UMAP visualization showing TAMs of individuals. (C) Top 20 function analysis results of TAM-1 cluster. Hypoxia-related biological processes were colored in red. (D) Violin plot of ERO1A expression level in different TAM clusters. (E) Immunofluorescence staining for TAM-1 cluster ( <t>CD14+ERO1A+</t> ) in patient tumor sample. The staining was performed for seven patients, one section each, and a representative image from patient 6 with TAM-1 pointed out by white arrows was shown; scale, 10 μm. The other images are shown in Supplementary <xref ref-type= Supplementary Figure 6 . (F) Composition ratio of the TAM clusters in individual GBM. (G) Correlation between the number of MES-like and TAM-1 cells. (H) Heatmap of specific gene sets in TAMs. (I) Violin plot of the hypoxia gene set scores in TAMs. TAM-1 was the hypoxia-response cluster in TAMs. (J) The activated regulons in the TAM-1 cluster ranked by the regulon specificity score from high to low. (K) Survival analysis of the TAM-1 cluster signatures in TCGA and CGGA GBM databases, respectively. Gray dash line, average score; **** p < 0.0001. See also Supplementary Figures 5 and 6 , and Supplementary Tables 3 and 6 . " width="250" height="auto" />
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    Image Search Results


    (A) Quantitative marker analysis showing percent of cells expressing CD73, CD105, CD14 and CD45 after long term expansion Synthemax II microcarriers in Mesencult XF and hMSC stemgro media. Flow cytometry histograms for each phenotypic marker are shown for cells maintained on Synthemax II microcarriers in Mesencult XF medium (B) and hMSC stemgro medium (C).

    Journal: PLoS ONE

    Article Title: Long Term Expansion of Bone Marrow-Derived hMSCs on Novel Synthetic Microcarriers in Xeno-Free, Defined Conditions

    doi: 10.1371/journal.pone.0092120

    Figure Lengend Snippet: (A) Quantitative marker analysis showing percent of cells expressing CD73, CD105, CD14 and CD45 after long term expansion Synthemax II microcarriers in Mesencult XF and hMSC stemgro media. Flow cytometry histograms for each phenotypic marker are shown for cells maintained on Synthemax II microcarriers in Mesencult XF medium (B) and hMSC stemgro medium (C).

    Article Snippet: Cells were incubated in blocking buffer for 15 min at 4°C, followed by 30 minute incubation at 4°C with primary antibodies against CD14, CD45, CD73, CD105 (Millipore Chemicon) or corresponding isotype controls (Invitrogen/Molecular Probes) in blocking buffer.

    Techniques: Marker, Expressing, Flow Cytometry

    Hypoxia-response TAMs in GBM. (A) UMAP visualization of TAMs. (B) UMAP visualization showing TAMs of individuals. (C) Top 20 function analysis results of TAM-1 cluster. Hypoxia-related biological processes were colored in red. (D) Violin plot of ERO1A expression level in different TAM clusters. (E) Immunofluorescence staining for TAM-1 cluster ( CD14+ERO1A+ ) in patient tumor sample. The staining was performed for seven patients, one section each, and a representative image from patient 6 with TAM-1 pointed out by white arrows was shown; scale, 10 μm. The other images are shown in Supplementary <xref ref-type= Supplementary Figure 6 . (F) Composition ratio of the TAM clusters in individual GBM. (G) Correlation between the number of MES-like and TAM-1 cells. (H) Heatmap of specific gene sets in TAMs. (I) Violin plot of the hypoxia gene set scores in TAMs. TAM-1 was the hypoxia-response cluster in TAMs. (J) The activated regulons in the TAM-1 cluster ranked by the regulon specificity score from high to low. (K) Survival analysis of the TAM-1 cluster signatures in TCGA and CGGA GBM databases, respectively. Gray dash line, average score; **** p < 0.0001. See also Supplementary Figures 5 and 6 , and Supplementary Tables 3 and 6 . " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Single-Cell Transcriptomics Revealed Subtype-Specific Tumor Immune Microenvironments in Human Glioblastomas

    doi: 10.3389/fimmu.2022.914236

    Figure Lengend Snippet: Hypoxia-response TAMs in GBM. (A) UMAP visualization of TAMs. (B) UMAP visualization showing TAMs of individuals. (C) Top 20 function analysis results of TAM-1 cluster. Hypoxia-related biological processes were colored in red. (D) Violin plot of ERO1A expression level in different TAM clusters. (E) Immunofluorescence staining for TAM-1 cluster ( CD14+ERO1A+ ) in patient tumor sample. The staining was performed for seven patients, one section each, and a representative image from patient 6 with TAM-1 pointed out by white arrows was shown; scale, 10 μm. The other images are shown in Supplementary Supplementary Figure 6 . (F) Composition ratio of the TAM clusters in individual GBM. (G) Correlation between the number of MES-like and TAM-1 cells. (H) Heatmap of specific gene sets in TAMs. (I) Violin plot of the hypoxia gene set scores in TAMs. TAM-1 was the hypoxia-response cluster in TAMs. (J) The activated regulons in the TAM-1 cluster ranked by the regulon specificity score from high to low. (K) Survival analysis of the TAM-1 cluster signatures in TCGA and CGGA GBM databases, respectively. Gray dash line, average score; **** p < 0.0001. See also Supplementary Figures 5 and 6 , and Supplementary Tables 3 and 6 .

    Article Snippet: The samples were incubated with the first primary antibody against CD14 (1:200 for IF, Servicebio) overnight at 4°C and then with the first corresponding secondary antibody at room temperature for 50 min under dark conditions.

    Techniques: Expressing, Immunofluorescence, Staining